Obesity reduces mitochondrial gene expression in fat tissue

Mitochondria are important cellular powerplants that process all of our energy intakes. If the pathways associated with breaking down nutrients are lazy, the changes can often have health-related consequences.

Helsinki: During a recent study, researchers at the Obesity Research Unit of the University of Helsinki found that obesity clearly reduces mitochondrial gene expression in fat tissue or adipose tissue.

Mitochondria are important cellular powerplants that process all of our energy intakes. If the pathways associated with breaking down nutrients are lazy, the changes can often have health-related consequences. The study was recently published in the journal Cell Reports Medicine.

A total of 49 pairs of identical twins discordant for bodyweight participated in the study conducted at the University of Helsinki: their body composition and metabolism were studied in detail, and biopsies from adipose and muscle tissue were collected. Multiple techniques for analysing the genome-wide gene expression, the proteome, and the metabolome were used in the study.

According to the findings, the pathways responsible for mitochondrial metabolism in adipose tissue were greatly reduced by obesity. Since mitochondria are key to cellular energy production, their reduced function can maintain obesity. For the first time, the study also compared the effects of obesity specifically on the mitochondria in muscle tissue in these identical twin pairs: muscle mitochondria too were found to be out of tune, but the change was less distinct than in adipose tissue.

The study provided strong evidence of a connection between the low performance of adipose tissue mitochondria and a proinflammatory state. Furthermore, the findings indicate that metabolic changes in adipose tissue are associated with increased accumulation of fat in the liver, prediabetic disorders of glucose and insulin metabolism as well as cholesterol.

“If mitochondria, the cellular powerplants, are compared to the engine of a car, you could say that the power output decreases as weight increases. A low-powered mitochondrial engine may also generate toxic exhaust fumes, which can cause a pro-inflammatory state in adipose tissue and, consequently, the onset of diseases associated with obesity,” says Professor Kirsi Pietilainen from the Obesity Research Unit, University of Helsinki.

“What was surprising was that the mitochondrial pathways in muscle had no association with these adverse health effects,” Pietilainen adds.

Obesity also affected amino acid metabolism

In the study, changes in mitochondrial function were also seen in amino acid metabolism. The metabolism of branched-chain amino acids, which are essential to humans, was weakened in the mitochondria of both adipose tissue and muscle tissue.

“This finding was of particular significance because the reduced breakdown of these amino acids and the resulting heightened concentration in blood has also been directly linked with prediabetic changes and the accumulation of liver fat in prior twin studies,” says Pietilainen.
Obesity, with its numerous associated diseases, is a common phenomenon that is continuously increasing in prevalence. While lifestyle influences the onset of obesity, genes also have a significant role.

“Identical twins have the same genes, and their weight is usually fairly similar. In fact, studying twins is the best way to investigate the interplay between genes and lifestyle. In spite of their identical genome, the genes and even mitochondria of twins can function on different activity levels. We utilised this characteristic in our study when looking into the effects of weight on tissue function,” Pietilainen says.