Akash Deep, who claimed 11 wickets in four games for India 'A' against England Lions last month, inflicted most of the damage with his impressive 3-24 spell on a crack-ridden pitch.
Rohit Sharma (131) and Ravindra Jadeja (112) struck fine hundreds while debutant Sarfaraz Khan made a promising start to his Test career with an impressive 62 off 66 balls.
The formidable partnership of 204 runs between captain Rohit and Jadeja firmly placed India in control on Day 1 of the ongoing third Test against England in Rajkot on Thursday.
However, miscommunication with star all-rounder Ravindra Jadeja in the middle cut short his lovely debut innings as he was run out by Mark Wood at the non-striker's end.
With India reduced to 33 for three inside the first hour of play, Rohit (131 off 196) and Jadeja (110 batting off 212) as India reached 326 for five at stumps on day one of the third Test against England on Thursday.
India were jolted early by Wood's express pace and movement off the surface which saw the home team's best batter in the series Yashasvi Jaiswal (10) walking back without contributing much.
Ahead of the third Test, players like Sarfaraz Khan and Dhruv Jurel were handed their Test caps.
English skipper Ben Stokes is to play his 100th Test match.
Iyer has complained of stiffness in the back and pain in the groin area and he Iyer will be sent to the National Cricket Academy (NCA) in Bangalore for further tests, an Indian Express report said.
Asked about his earlier remarks regarding Kohli, De Villiers, known for his strong bond with the former Indian captain off the field from their time at Royal Challengers Bangalore, altered his position.